EC Number |
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4.1.99.16 | expression in Escherichia coli |
4.1.99.16 | gene cyc2, recombinant expression of C-terminally His6-tagged full-length enzyme and of the N-terminal domain of ScGS (residues 1-366) in Escherichia coli strain BL21(DE3)pLysS , subcloning in Escherichia coli strain XL-1 Blue |
4.1.99.16 | gene geo, cloning of full-length of geosmin synthase gene from Aphanizomenon gracile strain WH-1, DNA and amino acid sequence determination and analysis, phylogenetic tree, real time PCR is applied to quantify the geosmin production in different light and temperature. Low temperature (15°C), high light intensity (0.035 mmol/s/m) and continuous light illumination are beneficial to the expression of geo. The successful amplification of geosmin synthase gene verifies that hiTAIL-PCR is an effective and simple procedure of low cost |
4.1.99.16 | gene sce1440, recombinant expression in Escherichia coli |
4.1.99.16 | relative enzyme expression analysis |