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Results 1 - 5 of 5
EC Number Cloned (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.16expression in Escherichia coli
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.16gene cyc2, recombinant expression of C-terminally His6-tagged full-length enzyme and of the N-terminal domain of ScGS (residues 1-366) in Escherichia coli strain BL21(DE3)pLysS , subcloning in Escherichia coli strain XL-1 Blue
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.16gene geo, cloning of full-length of geosmin synthase gene from Aphanizomenon gracile strain WH-1, DNA and amino acid sequence determination and analysis, phylogenetic tree, real time PCR is applied to quantify the geosmin production in different light and temperature. Low temperature (15°C), high light intensity (0.035 mmol/s/m) and continuous light illumination are beneficial to the expression of geo. The successful amplification of geosmin synthase gene verifies that hiTAIL-PCR is an effective and simple procedure of low cost
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.16gene sce1440, recombinant expression in Escherichia coli
Display the word mapDisplay the reaction diagram Show all sequences 4.1.99.16relative enzyme expression analysis
Results 1 - 5 of 5