EC Number   |
Reference |
|---|
   4.1.1.6 | espressed as recombinant protein in Escherichia coli |
708675 |
| 4.1.1.6 | expressed in Corynebacterium glutamicum strain ATCC13032 |
748549 |
| 4.1.1.6 | expressed in Escherichia coli BL21(DE3) cells |
749341 |
| 4.1.1.6 | expressed in Escherichia coli BL21(DE3) cells and in Aspergillus niger strain AB 1.13 |
715097 |
| 4.1.1.6 | expressed in Escherichia coli BW25113 (DE3) cells |
746886 |
| 4.1.1.6 | expressed in Escherichia coli BW25113 (DE3) pLysS cells |
747220 |
| 4.1.1.6 | expressed in Saccharomyces cerevisiae |
690578 |
| 4.1.1.6 | gene cadA, heterologous expression in Aspergillus niger strain N201, expression of two previously identified Aspergillus terreus genes encoding putative organic acid transporters, genes mttA and mfsA, increases itaconic acid production in an Aspergillus niger cis-aconitate decarboxylase expressing strain, production method optimization, overview |
726774 |
| 4.1.1.6 | gene cadA, heterologous functional expression in citric acid producing Aspergillus niger under various constitutive promoters of different expression strength, heterologous expression of the gene in Saccharomyces cerevisiae |
727576 |
| 4.1.1.6 | gene cadA, the cadA gene of is located in a gene cluster together with two transporters, one a putative mitochondrial carrier, the other a putative plasma membrane carrier, cloning in Escherichia coli strain TOP10, heterologous mitochondrial and cytosolic expression of the enzyme in Aspergillus niger strain ATCC 1015, the mitochondrial expression doubles the enzyme productivity compared to the cytosolic expression. Co-expression of a cytosolic or a mitochondrial aconitase with cytosolic CadA leads to a significant increase of itaconic acid production compared to cytsolic CadA expression alone. The rate-limiting step for itaconic acid production under these conditions appears to be both the provision of cis-aconitate as a substrate for the CadA as well as the CAD activity itself |
728240 |
