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EC Number
Cloned (Commentary)
4.1.1.33
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4.1.1.33
cloned under the control of the yeast PMA1 strong promoter, expression in Saccharomyces cerevisiae. Wild-type sterol content is not fully restored suggesting that the Arabidopsis thaliana enzyme activity may not be optimal in yeast
4.1.1.33
expressed in CV-1 cells
4.1.1.33
expressed in Escherichia coli BL21(DE3) cells
4.1.1.33
expressed in Escherichia coli BL21(DE3) cells. Whole cells of Eschercihia coli produced ScMDD with an N-terminal 6xHis tag form isobutene from 3-hydroxy-3-methylbutyrate at a rate 0.154 nmol/h * g cells
4.1.1.33
expressed in Saccharomyces cerevisiae
4.1.1.33
expression in Escherichia coli
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