EC Number |
---|
3.7.1.7 | expressed in Escherichia coli |
3.7.1.7 | expression in Escherichia coli under the control of the lac promoter |
3.7.1.7 | expression of the OPH gene lacking the sequence encoding the original signal peptide in Escherichia coli strain BL21 (DE3), the recombinant enzyme is secreted, productivity of recombinant OPH reaches 565.95 U/L/h without and 733.17 U/L/h in presence of 200 mM glycine. Subcloning in Escherichia coli strain JM109. Therecombinant extracellular enzyme inhibits growth of Escherichia coli cells |
3.7.1.7 | gene oph, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
3.7.1.7 | gene oph, recombinant methano-inducible expression of the enzyme, ligated into the pPIC9K vector behind the alpha-factor signal sequence, in Pichia pastoris strain GS115 |
3.7.1.7 | into the pETYSBLIC vector for expression in Escherichia coli BL21DE3 cells |
3.7.1.7 | recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 trxB (DE3) |
3.7.1.7 | recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |