3.5.5.8 | expression of the alpha, beta, and gamma subunits in Escherichia coli, the subunits assemble to an apo-heterododecamer (alphabetagamma)4 like the wild-type enzyme exhibiting no catalytic activity due to lack of bound Co2+ irrespective of the cobalt concentration in the medium, coepression of enzyme with activator protein P15K leads to a functional Co2+-bound enzyme which exhibits 78% of native enzyme activity, optimization of conditions, overview |
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