EC Number   |
|---|
   3.5.1.26 | DNA sequence determination and analysis, overexpression of mutant L15R in BHK and COS-1 cells, low expression level in endoplasmic reticulum, the recombinant active enzyme does not reach the lysosomes |
| 3.5.1.26 | expression in COS cells |
| 3.5.1.26 | expression in COS-1 cells and in BHK-21 cells |
| 3.5.1.26 | expression in Escherichia coli |
| 3.5.1.26 | expression in NIH-3T3 cells |
| 3.5.1.26 | gene AGA, DNA and amino acid sequence determination and analysis, genomic organization of Aurelia gene AGA and exon-intron structure, molecular phylogenetic analysis based on the nucleotide sequences of N-terminal nucleophile (Ntn)_asparaginase_2_like superfamily genes, quantitative real-time PCR enzyme expresssion analysis |
| 3.5.1.26 | gene AGA, DNA and amino acid sequence determination and analysis, sequence comparisons with human enzyme and Asobara tabida enzyme |
| 3.5.1.26 | gene AGA, DNA and amino acid sequence determination and analysis, sequence comparisons with human enzyme and Leptopilina heterotoma enzyme |
| 3.5.1.26 | gene AGA, genotyping, recombinant expression of codon-optimized genes encoding enzyme variants S149 and T149 in HEK-293T and HeLa cells, the Thr149 variant exhibits a slightly higher expression level than the Ser149 variant. Recombinant expression of AGA variants in transgenic patient fibroblasts, both wild-type and AGUFin-major mutant type. The transfection with the AGA variants improves the lysosomal morphology in AGU fibroblasts, low transfection efficiency |
| 3.5.1.26 | recombinant expression of wild-type and mutant enzymes |
