EC Number   |
|---|
   3.4.24.61 | - |
| 3.4.24.61 | expression of the wild-type enzyme and the mutant E235A in COS-7 or HEK-293T cells, co-expression with ADAM17 |
| 3.4.24.61 | full-length cDNA from a testis library |
| 3.4.24.61 | heterologous expression of hNRDc1 by both human myometrial smooth muscle cells and ltk- mouse fibroblasts. Analysation of the regulatory function of hNRDc1 regarding mK44 (a splice variant of maxi-K chanel). Data indicate that hNRDc1 is involved in regulating the excitation of smooth muscle cells. |
| 3.4.24.61 | isolation of cDNA |
| 3.4.24.61 | overexpression of V5-tagged NRDc from a a lentivirus vector in TACE-deficient fibroblasts, co-expression of NRDc with ADAM10, recombinant wild-type NRDc and mutant E235A are synthesized using a Bac-to-Bac baculovirus expression system, co-expression of the C-terminally V5-tagged wild-type enzyme with the TNF-alpha-converting enzyme in COS-7 cells |
| 3.4.24.61 | rat, testis |
| 3.4.24.61 | recombinant enzyme expressed in Sf9 cells |
