EC Number   |
Reference |
|---|
   3.4.21.34 | expressed in Drosophila S2 cells |
754520 |
| 3.4.21.34 | expressed in Pichia pastoris strain, X-33 |
753574 |
| 3.4.21.34 | expression of the protease domain in Pichia pastoris as a heterologously glycosylated zymogen that is activated by limited trypsin digestion and treated with endoglycosidase H deglycosidase to reduce heterogeneity from the glycosylation. In the baculovirus/Sf9 system, homogenous, crystallizable, and nonglycosylated protein is expressed after mutagenizing three asparagines to glutamate. The activity of the catalytic domain is similar to that of the full-length protein |
669411 |
| 3.4.21.34 | screening of genomic library, DNA and amino acid sequence determination and analysis |
653408 |
