EC Number   |
|---|
   3.4.17.22 | - |
| 3.4.17.22 | baculovirus expression of constructs containing either the 1A or the 1B form and one of three different sequences of domain 2 of CPD (wild type, mutants svr1, or svrpoi) in Sf9 cells |
| 3.4.17.22 | expressed by transient expression in a human kidney cell line and primary duck hepatocytes |
| 3.4.17.22 | expression in Sf9 cells, using a baculovirus expression system |
| 3.4.17.22 | human carboxypeptidase D (residues 32-1298) is cloned into the pTriExTM-7 expression vector that encodes an IgM secretion signal sequence and an N-terminal Strep-Tag II fusion protein. The CPD-pTrieX-7 construct ias transfected into mammalian HEK293F cells for transient expression, using polyethylenimine as transfection reagent |
| 3.4.17.22 | transfection of HEK-293F cell |
| 3.4.17.22 | wild-type enzyme and point mutants are expressed at high levels in Sf9 cells using the baculovirus expression system |
