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EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.B43- 656141
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.B43expression of a C-terminally truncated, recombinant keratanase-II in Escherichia coli. The truncated enzyme is designed to remove the domains predicted to be unnecessary for catalytic activity and detrimental to recombinant expression in Escherichia coli 750338
Display the word mapDisplay the reaction diagram Show all sequences 3.2.1.B43synthetic truncated keratanase II gene is subcloned into the NdeI and XhoI sites of pET30a, resulting in the expression construct pKSII, which placed a His6 tag at the extreme C-terminus of the enzyme, expression in Escherichia coli BL21 750715
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