EC Number   |
|---|
   3.2.1.83 | DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain DH5alpha as extracellular enzyme |
| 3.2.1.83 | expressed in Brevibacillus choshinensis |
| 3.2.1.83 | expressed in Escherichia coli BL21(DE3) cells |
| 3.2.1.83 | expressed in Escherichia coli C43(DE3) cells |
| 3.2.1.83 | expressed in Pichia pastoris GS-115 cells |
| 3.2.1.83 | expression in Escherichia coli |
| 3.2.1.83 | gene cgkA, recombinant expression of functional CBM-tagged chimeric fusion enzyme cCgkA-cCglA, consisting of the catalytic domains of kappa-carrageenase CgkA and lambda-carrageenase CglA from Pseudoalteromonas carrageenovora fused with a dockerin domain, in Escherichia coli strain BL21(DE3), cloning in Escherichia coli strain DH5alpha |
| 3.2.1.83 | gene cgkZ, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of His-tagged enzyme with and without signal peptide extracellulary and intracellularly in Escherichia coli strain BL21 (DE3), cloning in Escherichia coli strain DH5alpha |
| 3.2.1.83 | gene cgkZ, recombinant expression of N-terminally His6-tagged extracellular enzyme in Escherichia coli strain BL21(DE3), 51% of the total kappa-carrageenase is secreted into culture medium, and 33% accumulates in the periplasmic space. Low temperature of 23°C and optimal isopropyl-beta-L-thiogalactoside concentration of 0.9 mM favor soluble protein expression, additives such as lactose, glycine, Tween-80, and Triton X-100 promote the release of intracellular enzymes, method optimization and evaluation, overview |
| 3.2.1.83 | His-tagged enzyme expression in the periplasm of Escherichia coli strain BL21(DE3) |
