EC Number   |
|---|
   3.2.1.63 | - |
| 3.2.1.63 | expressed in Escherichia coli BL21(DE3) cells |
| 3.2.1.63 | expression of 1,2-alpha-L-fucosidase in Escherichia coli, expression of the Fuc domain (responsible for fucosidase activity) as a hexahistidine-tagged protein in Escherichia coli |
| 3.2.1.63 | gene afcA, expression in Escherichia coli and in enzyme-deficient Bifidobacterium longum strain 105-A which renders the transformant able to utilize 2'-fucosyllactose as the sole carbon source |
| 3.2.1.63 | overexpressed in Escherichia coli as 6×His-tagged protein |
| 3.2.1.63 | overexpression in Escherichia coli, selenomethionine-substituted protein |
| 3.2.1.63 | sequence comparisons, recombinant enzyme expression of the enzyme including its native signal peptide from pPICZAalpha without the C-terminal Myc/His6 tags in Pichia pastoris, expression of the His6-tagged crystallin domain, residues 501-585, of the enzyme fused to maltose-binding protein at the N-terminus in Escherichia coli |
| 3.2.1.63 | wild-type and mutant derivatives of the catalytic domain of AfcA (Fuc domain) |
