EC Number   |
|---|
   3.2.1.149 | - |
| 3.2.1.149 | cloned from tea flowers, recombinant inducible expression of His-tagged enzyme in Escherichia coli |
| 3.2.1.149 | expression in Escherichia coli |
| 3.2.1.149 | full-length cDNA, including a putative N-terminal secretion signal sequence, cloned into the baculovirus expression plasmid, expressed in Sf9 cells or High-five cells |
| 3.2.1.149 | phylogenetic analysis and tree |
| 3.2.1.149 | quantitative RT-PCR expression analysis of the enzyme is wild-type and transgenic plant leaves, recombinant expression of GFP-tagged enzyme in transgenic Nicotiana benthamiana plants via transfection method with Agrobacterium tumefaciens |
| 3.2.1.149 | sequence comparison, the enzyme including the N-terminal signal sequence is cloned and expressed in a baculovirus-insect cell system |
