EC Number   |
|---|
   3.2.1.140 | Bifidobacterium bifidum grown anaerobically in GAM medium, enzyme gene inserted into plasmid pMW118, expression of truncated form missing signal peptide and membrane anchor (era 35-1064) in Escherichia coli rosetta(DE3) pLacI with plasmid pET23b-lnbB, LB medium, 37°C |
| 3.2.1.140 | C-terminally His6-tagged enzyme |
| 3.2.1.140 | cloned in Escherichia coli Rosetta(DE3) |
| 3.2.1.140 | gene lnbB, expression of His-tagged wild-type enzyme in Escherichia coli BL21 CodonPlus (DE3)-RIL and of His-tagged selenomethionine-labeled enzyme in Escherichia coli BL21 CodonPlus (DE3)-RIL X |
| 3.2.1.140 | gene lnbX, genomic library construction of Bifidobacterium longum strain JCM1217 in Escherichia coli strain DH5alpha, gene disruption and complementation analysis, recombinant expression of lnbX in non-tagged and C-terminally His-tagged form, coexpression with N-terminall His-tagged LnbY in Escherichia coli. The addition of His-tag to the C-terminus of the protein does not alter the enzymatic properties of LnbX |
| 3.2.1.140 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 3.2.1.140 | recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Star |
