Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search Cloned(Commentary)

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 4 of 4
EC Number Cloned (Commentary)
Display the reaction diagram Show all sequences 3.1.3.72expression in Pichia pastoris
Display the reaction diagram Show all sequences 3.1.3.72gene appA or phyS, cloning from library, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of extracellular His-tagged enzyme in Escherichia coli strain BL21(DE3) as non-glycosylated protein and of extracellular nontagged enzyme in Pichia pastoris strain GS115(his4) under control of the AOX1 promoter as glycosylated protein
Display the reaction diagram Show all sequences 3.1.3.72gene phyAsrl, DNA and amino acid sequence determination and analysis, phyAsrl is constitutively expressed
Display the reaction diagram Show all sequences 3.1.3.72recombinant extracellular expression of Lilium longiflorum alkaline phytase in Pichia pastoris strain X33. For analysis of the influence of the native signal sequences on alpha-MF-directed secretion, three expression constructs are generated by inserting three forms of rLlAlp2 downstream of the alpha-MF secretion signal in pPICZaAvectors: 1. full length optimized rLlAlp2 sequence (pPICZaA-op-rLlAlp2), 2. truncated oprLlAlp2 without QKTEL at the C-terminus (pPICZaA-op-LlAlp2DC), and 3. truncated op-rLlAlp2 without the first 19 aa at the N-terminus and without QKTEL at the C-terminus (pPICZaA-oprLlAlp2DNDC). By switching from the strong, tightly regulated promoter PAOX1 to the weaker, constitutive promoter PGAP and optimizing culture conditions, an 8 to 10fold increase in extracellular expression of rLlALP2 is achieved, optimization of culture conditions for enhanced expression, method evaluation, overview
Results 1 - 4 of 4