EC Number |
---|
3.1.21.7 | - |
3.1.21.7 | expressed in a modified version of Escherichia coli ER 2566 (ER2566DELTAnfiDELTArnhB) where the endogenous nfi and rnhB genes have been inactivated by transformation with the kanamycin-deletion cassettes derived from the JW5547-1 (nfi::kan) and JW0178-1 (rnhB::kan) strains. For crystallisation, a site specific (D52A) truncated (residues 1-250) mutant of mEndoV is fused to His/MBP in pETM41 is expressed in BL21 CodonPlus(DE3)-RIPL Escherichia coli cells |
3.1.21.7 | expressed in Escherichia coli BL21(DE3) cells |
3.1.21.7 | expressed in Escherichia coli BL21-Codon Plus (DE3)-RIPL cells |
3.1.21.7 | expressed in Escherichia coli strain BL21 CodonPlus (DE3) RIL |
3.1.21.7 | expression in Escherichia coli |
3.1.21.7 | expression in Escherichia coli BL21 (DE3) |
3.1.21.7 | expression in Escherichia coli GWR109 |
3.1.21.7 | HEK 293T cells are transiently transfected with a vector expressing GFP-hEndoVwt |
3.1.21.7 | overexpression in Escherichia coli |