EC Number   |
|---|
   2.8.2.14 | expressed in Escherichia coli BL21(DE3) cells |
| 2.8.2.14 | expressed in HEK-293 cells |
| 2.8.2.14 | expressed in HEK293 cells |
| 2.8.2.14 | expression in HepG2 cells or CaCo-2 cells |
| 2.8.2.14 | gene SULT2A1, recombinant expression |
| 2.8.2.14 | gene Sult2a8 is localized to chromosome 7, embedded in a 0.9 Mb region harboring the cluster of Sult2a genes (Sult2a5-Sult2a2-Sult2a1-Sult2a4-Sult2a3-Sult2a6-Sult2a8-Sult2a7), as well as several Sult2a pseudogenes |
| 2.8.2.14 | gene Sult2a8, DNA and amino acid sequence determination and analysis, mL-STL cDNAs exhibit multiple 5' termini and skipping of nucleotides, sequence analysis of 12 mL-STL 3'-RACE clones. The mL-STL gene has multiple alternative exonic splicing variants, long and short forms of mL-STL cDNAs. The long-form mL-STL gene is composed of seven exons separated by six introns, with a total gene size of 35902 bp. Recombinant expression of non-tagged and His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS, half of the expressed protein is soluble, whereas the remaining protein is misfolded to form inclusion bodies |
| 2.8.2.14 | gene Sult2a8, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of GST-tagged wild-type and mutant enzymes in Escherichia ocli strain BL21 |
