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EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22- 761944
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22cloning of the junctions of the integrated Tgf2 cis-element and the surrounding genomic DNA using inverse PCR. Four EGFP-positive blunt snout bream are sampled from the group coinjected with donor plasmid with recombinant Tgf2 TPase. An 8-bp direct repeat of target DNA at the integration site, the signature of hAT family transposons, is created adjacent to both ends of Tgf2 at the integration sites in all fish, indicating the recombinant Tgf2 TPase insertions occur by transposition, recombinant expression of soluble His6-tagged enzyme in Escherichia coli strain Rossetta 1 (DE3) 739044
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22DNA sequence determination and analysis of several IS1341-type transposases (tnpB genes), existence of sense overlapping transcripts (sotRNAs) for all IS1341-type transposases in Halobacterium salinarum strain NRC-1, condition-dependent differential regulation between sotRNAs and their cognate genes. Identification of a UUCA tetraloop motif that is present in other archaea (ncRNA family HgcC) and in a Halobacterium salinarum intergenic ncRNA derived from a palindrome associated transposable elements (PATE). Overexpression of one sotRNA and the PATE-derived RNA harboring the tetraloop motif improves Halobacterium salinarum growth, indicating that these ncRNAs are functional. IS1341-type transposase genes harbor the right end (RE) of the IS element, which may explain the sotRNA motifs found as a retained structure formed at DNA level, also stable at RNA level, i.e. the RE-like tetraloop 739632
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22expression in Acidithiobacillus ferrooxidans 760393
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22expression in Escherichia coli 760785, 761966, 762411
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22expression in HEK-293 and HeLa cells and in mice 762040
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22expression in HeLa cell 761863
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22expression of wild-type and mutant enzyme Tgf2TPase and transposons in Escherichia coli strain Rosetta1 (DE3) 739693
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22full-length Hermes expressed in eukaryotic cells, such as budding yeast and Sf9 cells, similarly forms a large multimeric species consistent with an octamer as judged by gel filtration 738023
Display the word mapDisplay the reaction diagram Show all sequences 2.7.7.B22Galileo gene structure comparisons in Drosophila species, overview 738320
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