EC Number |
---|
2.7.7.86 | - |
2.7.7.86 | cloning from HeLa cells and analysis of the cGAS promoter region (-414 to +76 relative to the transcription start site), which is involved in the molecular mechanisms controlling the cGAS transcriptional activity, it is sufficient for promoter activity, RT-PCR enzyme expression analysis, transcription start site (TSS) localization of human cGAS (hcGAS) promoter |
2.7.7.86 | expressed in Escherichia coli Rosetta2 (DE3) cells |
2.7.7.86 | expressed in HEK-293 cells |
2.7.7.86 | expressed in HEK293T cells |
2.7.7.86 | expressed in HEK293T cells and expressed in Escherichia coli as a SUMO (small ubiquitin-related modifier protein) fusion protein |
2.7.7.86 | gene CGAS, quantitative real-time PCR enzyme expression analysis |
2.7.7.86 | gene CGAS, recombinant expression of isolated His-tagged h-cGAS catalytic domain in Escherichia coli strain Rosetta 2(DE3) |
2.7.7.86 | gene MB21D1, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic analysis, quantitative real-time PCR analysis. Recombinant expression of FLAG-tagged cGAS in CEF cells |