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EC Number Cloned (Commentary)
Display the reaction diagram Show all sequences 2.7.1.174expressed in Escherichia coli BL21(DE3) cells
Display the reaction diagram Show all sequences 2.7.1.174gene DGK1
Display the reaction diagram Show all sequences 2.7.1.174gene DGK1, the DGK1 promoter is substituted with the inducible GAL1/10 promoter in the low copy YCplac111 and high copy YEplac181 vectors, expression of wild-type and mutant enzymes, induction of wild-type DGK1 gene expression from high copy number plasmid YEplac181-GAL1/10-DGK1 results in a massive increase in DAG kinase activity. Temperature sensitivity of dgk1DELTA cells overexpressing DGK1 and its mutant alleles
Results 1 - 3 of 3