EC Number   |
|---|
    2.5.1.81 | - |
| 2.5.1.81 | expressed in Escherichia coli as a glutathione S-transferase fusion protein |
| 2.5.1.81 | expression of wild-type and mutant enzymes in Escherichia coli |
| 2.5.1.81 | gene AtIDS1, recombinant overexpression of GST-tagged enzyme in Escherichia coli strain BL21, and recombinant enzyme expression in the loss-of-function AtIDS11 mutant ggpps11-4, which has an embryolethal phenotype due to the lack of GGDP. AtIDS1, a predominantly GFDP-producing enzyme that nonetheless forms a sizable amount of GGDP, complements the embryo-lethal phenotype of ggpps11-4. Transient expression of N-terminally GFP-tagged enzyme in Nicotiana benthamiana |
| 2.5.1.81 | gene AtIDS10, recombinant overexpression of GST-tagged enzyme in Escherichia coli strain BL21, transient expression of N-terminally GFP-tagged enzyme in Nicotiana benthamiana |
| 2.5.1.81 | gene AtIDS6, recombinant overexpression of GST-tagged enzyme in Escherichia coli strain BL21, transient expression of N-terminally GFP-tagged enzyme in Nicotiana benthamiana |
| 2.5.1.81 | gene AtIDS7, recombinant overexpression of GST-tagged enzyme in Escherichia coli strain BL21, transient expression of N-terminally GFP-tagged enzyme in Nicotiana benthamiana |
| 2.5.1.81 | gene AtIDS9, recombinant overexpression of GST-tagged enzyme in Escherichia coli strain BL21 and of N-terminally His6-tagged enzyme in Arabidopsis thaliana ecotype Col-0 under control of CaMV 35S promoter via Agrobacterium tumefaciens strain GV3101 transfection method, and recombinant enzyme expression in the loss-of-function AtIDS11 mutant ggpps11-4, which has an embryolethal phenotype due to the lack of GGDP. Transient expression of N-terminally GFP-tagged enzyme in Nicotiana benthamiana |
| 2.5.1.81 | gene Lc-GFDPS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant expression in Escherichia coli, and in Arabidopsis thaliana driven by a double CaMV 35S promoter |
