2.5.1.148 | recombinant expression of the enzyme in Escherichia coli, LOS coexpression in Saccharomyces cerevisiae with Arabidopsis thaliana GGPP synthase-11 (AtGGPPS11) resulting in lycopaoctaene production, which is undetectable when AtGGPPS11 is expressed without LOS. Recombinantly expressed in a Saccharomyces cerevisiae SS knockout strain, LOS restores ergosterol prototrophy, indicating its ability to produce squalene in vivo |