EC Number   |
|---|
    2.4.2.26 | - |
| 2.4.2.26 | 5' and 3' deletion constructs expressed in SW1353 cells |
| 2.4.2.26 | c-Myc/TGFalpha doubly transgenic mice. Modified minigene Xylt2 expression construct producing a protein lacking the 45 N-terminal amino acids including the transmembrane domains. The Xylt1 expression cassette producing a protein that lacks 94 N-terminal amino acids including the transmembrane domain which is predicted as for XylT2. Both Xylt1 and Xylt2 expression cassettes cloned into a modified pcDNA3.1 vector containing a transferrin signal peptide and an HPC4 epitope tag in frame on the N-terminus |
| 2.4.2.26 | DNA sequence determination and analysis of isoforms XT-I and XT-II, chromosome mapping |
| 2.4.2.26 | DNA sequence determination and analysis, chromosome mapping, functional expression of isoforms XT-I and XT-II in CHO-K1 cells, no activity when XT-II is expressed fused to the aminoterminal peptide tag, 8% of the recombinant activity is located in the cytosol and membranes, 92% recombinant activity is secreted into the medium |
| 2.4.2.26 | expressed as glutathione-S-transferase fusion proteins containing putative or known GAG attachment sites of in vivo proteoglycans. Cloned into the XhoI and EcoRI digested pGEX-6P-1 vector and ligated by T4 DNA ligase. Expressed in Escherichia coli BL21 DE3 cells |
| 2.4.2.26 | expressed in CHO cells, high five insect cells, HEK293 cells, and SaOS-2 cells, and in Pichia pastoris |
| 2.4.2.26 | expressed in Pichia pastoris strain X-33 |
| 2.4.2.26 | expressed in the xylosyltransferase-deficient pgsA-745 (S745) CHO cell line and in Pichia pastoris |
| 2.4.2.26 | expressed in xylosyltransferase-deficient pgsA-745 CHO cells |
