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EC Number Cloned (Commentary) Reference
Show all pathways known for 2.4.1.257Display the reaction diagram Show all sequences 2.4.1.257gene ALG2, cloning from Saccharomyces cerevisiae strain W303a, recombinant expression of wild-type and mutant N-terminal thioredoxin (Trx)-His6-tagged enzymes in Escherichia coli strain DE3 759165
Show all pathways known for 2.4.1.257Display the reaction diagram Show all sequences 2.4.1.257gene ALG2, transient recombinant expression of N-terminally His10-YFP tagged enzyme in HEK-293 cells 759307
Show all pathways known for 2.4.1.257Display the reaction diagram Show all sequences 2.4.1.257overexpression in Escherichia coli. Two Alg2 constructs are expressed and isolated, one with the N-terminal TRX domain and C-terminal His and V5 epitope tags and the other with only an N-terminal His tag 707472
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