EC Number |
---|
2.4.1.248 | - |
2.4.1.248 | expressed in Escherichia coli |
2.4.1.248 | expression in Escherichia coli |
2.4.1.248 | gene cit, DNA and amino acid sequence determination and analysis, expression in Escherichia coli |
2.4.1.248 | gene cit, expression in Escherichia coli |
2.4.1.248 | overexpression of CITase in Bacillus circulans strain G22-10, and, with the alpha-amylase promoter (PamyQ) and amyQ signal sequence of Bacillus amyloliquefaciens, in a protease-deficient Bacillus subtilis strain 168 as extracellular protein. The Bacillus subtilis host-vector system allows production of cycloisomaltooligosaccharides by direct fermentation of dextran along with high CITase production, which is not possible in Bacillus circulans G22-10 due to growth inhibition by dextran at high concentrations and limited production of CITase, stable expression up to 48 h |
2.4.1.248 | the C-terminal region of TtCITase (TtCITase-C, Met740-Phe1559), including a CBM35-like region and the GH family 15 domain. The domain is successfully cloned, expressed, and purified |