EC Number   |
|---|
   2.4.1.210 | cDNA clone encoding limonoid UDP-glucosyltransferase isolated, single copy gene in the citrus genome |
| 2.4.1.210 | Citrus unshiu is heterozygous posxadsessing both alleles, CitLGT1/CitLGT2, two alleles (CitLGT1/ CitLGT2) of LGT at a single locus |
| 2.4.1.210 | CrLGT cloning of full-length gene from a cDNA library, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, cloning in Escherichia coli strain DH5alpha, RT-PCR enzyme expression analyxadsis |
| 2.4.1.210 | expression in Escherichia coli for sequencing |
| 2.4.1.210 | gene SLLGT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli and in Pichia pastoris |
| 2.4.1.210 | gene SLLGT, single copy gene encoding LGT |
| 2.4.1.210 | recombinant expression of the MBP-tagged enzyme (MBP-cuGT) in Escherichia coli strain BLR(DE3). MBP-cuGT does not exhibit any detectable effect on limonin degradation when Navel orange juice is used as the substrate. MBP-cuGT is unable to biotransform either limonoate A-ring lactone (LARL) or limonoate as purified substrates. But MBP-cuGT displays a broad activity spectrum towards flavonoids, confirming that the enzyme produced is active under the conditions evaluated in vitro, expression method evaluation, overview |
| 2.4.1.210 | single copy gene encoding LGT |
| 2.4.1.210 | single copy gene encoding LGT, Citrus sinensis is homozygous for allele CitLGT1 |
