EC Number   |
Reference |
|---|
    2.4.1.131 | - |
710691 |
| 2.4.1.131 | ALG9 gene encodes a middle-arm terminal alpha1,2-mannosyltransferase Alg9p |
638572 |
| 2.4.1.131 | construction of a plasmid pREP1+/-ALG11HA for the expression of Saccharomyces cerevisiae ALG11 in Schizosaccharomyces pombe and introducing it into gmd3 mutant. The gmd3 mutant, carrying pREP+/-ALG11HA, grows at 37°C, whereas the gmd3 mutant, carrying vector pREP1 alone, does not. The defect in acid phosphatase glycosylation of gmd3 mutant is also suppressed by Saccharomyces cerevisiae ALG11. The gmd3+/alg11+ gene is a functional homologue of the Saccharomyces cerevisiae ALG11 gene |
710691 |
| 2.4.1.131 | gene ALG11, recombinant expression of N-terminally His10-tagged enzyme in Escherichia coli strain BL21-Gold (DE3) |
759307 |
| 2.4.1.131 | overexpression in Escherichia coli. Two Alg2 constructs are expressed and isolated, one with the N-terminal TRX domain and C-terminal His and V5 epitope tags and the other with only an N-terminal His tag |
707472 |
| 2.4.1.131 | retroviral expression of human wild-type and mutated ALG11 cDNA in patient-derived fibroblasts as well as using a yeast alg11 deletion strain as a heterologous expression system for hALG11 variants |
708657 |
| 2.4.1.131 | transformation of the LEW3 gene into the yeast mutant alg11, which exhibits a lowtemperature-sensitive phenotype and grows slowly at and below 30°C but normally at 37°C. Expression of LEW3 partially complements the slow-growth phenotype of the yeast mutant alg11, suggesting that the plant ALG11 homolog is able to partially substitute for the function of ALG11 in yeast |
710284 |
