EC Number |
---|
2.3.3.14 | - |
2.3.3.14 | expressed at high levels in Escherichia coli |
2.3.3.14 | expressed in Escherichia coli B834 DE3 cells |
2.3.3.14 | expressed in Escherichia coli BL21(DE3) RIL star cells |
2.3.3.14 | expressed in Escherichia coli BL21(DE3) Rosetta 2 cells |
2.3.3.14 | expressed in Escherichia coli Rosetta (DE3) pLysS cells |
2.3.3.14 | expression in Escherichia coli |
2.3.3.14 | expression in Escherichia coli. Introduction of homocitrate synthase, homoaconitase and homoisocitrate dehydrogenase from Saccharomyces cerevisiae into Escherichia coli enables +1 carbon extension from 2-oxoglutarate to 2-oxoadipate, which is subsequently converted into glutarate by a promiscuous 2-oxo acid decarboxylase (KivD) and a succinate semialdehyde dehydrogenase (GabD) |
2.3.3.14 | into the parallel expression vector pHIS2 |
2.3.3.14 | overexpression in Penicillium chrysogenum using additional copies of lys1 with its own promoter or under the control of the pcbC promoter in either autonomously replicating or integrative vectors. Tranformants containing 3 to 32 additional copies of the lys1 gene are selected |