EC Number   |
|---|
    2.3.1.94 | a variant of DEBS1 is cloned into the vector pET21c |
| 2.3.1.94 | expression in Bacillus subtilis |
| 2.3.1.94 | expression in Escherichia coli |
| 2.3.1.94 | for expression in Escherichia coli cells |
| 2.3.1.94 | heterologous expression in Streptomyces coelicolor and Escherichia coli |
| 2.3.1.94 | into the vector pET21c |
| 2.3.1.94 | the ACP construct is engineered with the FLAG epitope |
| 2.3.1.94 | the acyltransferase domains, AT1, AT2, AT4, AT5 and AT6, from DEBS are expressed as stand-alone proteins with their ketosynthase-to-acyltransferase and post-acyltransferase linkers, ketosynthase3-acyltransferase3 and ketosynthase6-acyltransferase6 didomains and discrete acyl carrier protein domain expression vectors are constructed, the vector pET28a is used for expression in Escherichia coli BL21DE3 cells |
| 2.3.1.94 | the thioesterase domain from deoxyerythronolide B synthase is cloned for expression in Escherichia coli cells |
| 2.3.1.94 | the three DEBS genes are sequentially integrated into the Escherichia coli BAP1 chromosome |
