EC Number   |
|---|
    2.3.1.65 | cDNA encoding enzyme is cloned, sequenced and expressed in Escherichia coli DH5alpha, the open-reading frame codes for a 420 amino acid protein, Baat gene is mapped to mouse chromosome 4, single gene encodes enzyme |
| 2.3.1.65 | expressed in Escherichia coli DH5alpha cells |
| 2.3.1.65 | expression in Escherichia coli |
| 2.3.1.65 | expression in Sf9 cells |
| 2.3.1.65 | expression in Sf9 cells. Initial attempts to express rBAT in Escherichia coli with vectors such as pMAL-p2 and pKK233-2 are unsuccessful due to proteolytic degradation of the expressed protein |
| 2.3.1.65 | expression of GFP-tagged enzyme in HeLa cells, which is exclusively located in the cytosol, high mobility of the GFP-fusion proteins in the cytosol and absence of peroxisomal escape |
| 2.3.1.65 | expression of wild-type and mutant enzymes |
| 2.3.1.65 | gene/full-length cDNA is cloned and expressed in Escherichia coli XL1-Blue or BL21 |
| 2.3.1.65 | when expressed in fibroblasts, GFP-tagged hBAAT localizes to the cytosol |
