EC Number   |
Reference |
|---|
    2.3.1.54 | - |
14931, 487228, 487237 |
| 2.3.1.54 | Escherichia coli SP 264 contains plasmid p29with pfl gene, identification of pfl gene product as a 80kDa polypeptide |
14936 |
| 2.3.1.54 | expressed in Escherichia coli BL21(DE3), pET9a expression vector, transformation into pyruvate formate-lyase-deficient Escherichia coli strain BL21, capacity for formate excretion shown by in vitro tests |
686477 |
| 2.3.1.54 | expressed in Escherichia coli DC10B cells |
757504 |
| 2.3.1.54 | gene deletion is transduced via Bacteriophage P1 |
701826 |
| 2.3.1.54 | gene pfl from strain JM109, DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes in strain BL21(DE3) |
672694 |
| 2.3.1.54 | gene pfl, DNA and amino acid sequence determination and analysis, detailed phylogenetic analysis, overview |
674782 |
| 2.3.1.54 | gene pfl, DNA and amino acid sequence determination and analysis, sequence comparison |
672873 |
| 2.3.1.54 | gene pflB, coexpression of pyruvate formate lyase with the pyruvate formate lyase activating enzyme (gene pflA, UniProt ID P0A9N4) as well as with appropriate electron donors flavodoxin and ferredoxin (encoded by genes fldA and fdx, respectively), in a non-ethanol-producing Saccharomyces cerevisiae strain IMI076 lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1, IMI076 (Pdc- MTH1-DELTAT ura3-52), plasmid maps, overview. Reduced flavodoxin is the preferred electron donor, but coexpression of either of the electron donors has a positive effect on growth under aerobic conditions. Subcloning in Escherichia coli strain DH5alpha. The PFL pathway can be functional at aerobic growth conditions in yeast when coexpressed with appropriate electron donors |
744943 |
| 2.3.1.54 | gene pflB, enzyme expression analysis |
736361 |
