EC Number |
---|
2.3.1.32 | expressed as a His-tagged fusion protein |
2.3.1.32 | nuclear enzyme overexpression in HEK-293 cells |
2.3.1.32 | PCR-amplification, stable transfection of CHO cells, with myc-tag, overexpressing enzyme |
2.3.1.32 | recombinant p300 is expressed in baculovirus, and K1358A HAT mutant gene is expressed in Escherichia coli BL21 |
2.3.1.32 | recombinant PCAF expressed in baculovirus |
2.3.1.32 | transfection of COS-1 cells (African green monkey cell line) with plasmids of wild-type enzyme, deficient enzyme or single site mutants fused to reporter genes |