EC Number |
---|
2.3.1.28 | - |
2.3.1.28 | cloning of enzyme form CATB7 |
2.3.1.28 | cloning of mutant cat-86 in pTB361 and transformation of Escherichia coli JM109 |
2.3.1.28 | expressed in Escherichia coli |
2.3.1.28 | gene cat, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21-CodonPlus(DE3)-RIPL and in Geobacillus kaustophilus strain MK480 |
2.3.1.28 | overexpression in Escherichia coli harboring the plasmid pYT1 |
2.3.1.28 | PCR-amplification, clinging into Escherichia coli MC1061, electrotransformaion of Leuconostoc mesenteroides DRC (isolated from fermenting salted Chinese cabbage/Kimchi) |
2.3.1.28 | PCR-amplification, electrotransformation into Escherichia coli DH10B to test for chloramphenicol inactivation with Micrococcus lutes ATCC 9341 as indicator organism |
2.3.1.28 | plasmid Rms418 is transferred from Vibrio anguillarum to Escherichia coli K-12 |
2.3.1.28 | recombinant expression in Escherichia coli strain BL21(DE3) |