EC Number   |
|---|
   2.3.1.168 | cloning of full length E2 and fragments, expression in Escherichia coli JM109 as fusion protein |
| 2.3.1.168 | DNA and amino acid sequence determination anad analysis |
| 2.3.1.168 | DNA sequence determination, overexpression of wild-type, lipol-free apo-enzyme, and mutants in Escherichia coli |
| 2.3.1.168 | expressed as recombinant protein in Escherichia coli |
| 2.3.1.168 | expression in Escherichia coli |
| 2.3.1.168 | expression of apo-enzyme in Escherichia coli strain XL1-Blue, can be lipoylated in vitro |
| 2.3.1.168 | expression of E2c, residues 161-421, fused to maltose-binding protein, which enhances the yield of the recombinant enzyme, in Escherichia coli |
| 2.3.1.168 | gene contains a mitochondrial targeting presequence |
| 2.3.1.168 | gene E2, DNA sequence determination and analysis |
| 2.3.1.168 | gene E2, mapping to chromosome 1p31 |
