EC Number   |
|---|
    2.3.1.133 | - |
| 2.3.1.133 | an antisense construct is generated for down-regulation of HCT |
| 2.3.1.133 | expressed in Escherichia coli |
| 2.3.1.133 | expression in Escherichia coli |
| 2.3.1.133 | expression of GST-tagged HCT in Escherichia coli strain BL21 (DE3) |
| 2.3.1.133 | gene cbhct2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged HST in Escherichia coli strain BL21(DE3)pLysS |
| 2.3.1.133 | gene OsHCT4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as soluble GST-tagged enzyme using vector pGEX 5X-1 in Escherichia coli strain BL21(DE3) |
| 2.3.1.133 | genes PeHCT-1, PeHCT-2, PeHCT-3, PeHCT-4, PeHCT-5, PeHCT-6, and PeHCT-7, expression as GST-tagged enzymes in Escherichia coli strain BL21 (DE3) |
| 2.3.1.133 | overexpression of N-terminally His6-tagged HCT in Escherichia coli strain BL21 Star(DE3)pLysS using a commercially available synthetic gene encoding HCT with codons optimized for Escherichia coli expression |
| 2.3.1.133 | recombinantly expressed in Escherichia coli |
