EC Number   |
|---|
   2.3.1.107 | enzyme is expressed as recombinant His-tagged protein in Escherichia coli |
| 2.3.1.107 | expressed in Escherichia coli and Catharanthus roseus |
| 2.3.1.107 | expression in Escherichia coli |
| 2.3.1.107 | gene Dat, DNA sequence determination and analysis |
| 2.3.1.107 | the CrDAT transgene is transferred into Catharanthus roseus by Agrobacterium-mediated transformation and generated transgenic periwinkle lines with an increase in vincristine accumulation. The Catharanthus roseus DAT gene is introduced into Catharanthus roseus plants and it ias confirmed that CrDAT is successfully transferred into the genome of periwinkle plants and efficiently translated to synthesise recombinant DAT protein |
| 2.3.1.107 | transformation to Agrobacterium tumefaciens |
| 2.3.1.107 | transient expression of N-terminally or C-terminally GFP-tagged DAT in Catharanthus roseus cells. Irrespective to the fusion orientation, DAT is localized to the cytoplasm and the nucleus, as highlighted by the perfect overlapping of the fluorescence signal of the fusion protein with the nucleocytoplasmic CFP marker |
