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EC Number	Cloned (Commentary)
2.1.1.308	-
2.1.1.308	cloning and characterization of a complete fosfomycin biosynthetic cluster from Streptomyces fradiae and heterologous production of fosfomycin in Streptomyces lividans
2.1.1.308	overexpression in Escherichia coli Rosetta 2 (DE3) pLysS cells as a hexahistidine-tagged protein. The recombinant proteins are found exclusively in inclusion bodies. After refolding and concentration, the protein is reconstituted with additional iron and sulfide to maximize cluster formation

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Release 2023.1 (January 2023)