EC Number   |
|---|
   2.1.1.248 | gene mtmB |
| 2.1.1.248 | gene mtmB, DNA and amino acid sequence determination and analysis, genetic organization, the open reading frame of mtmB is interrupted by a single in-frame, midframe, UAG codon. A mechanism that circumvents UAG-directed termination of translation must operate during expression of mtmB in this methanogen. Analysis of the clustered genes encoding the methyltransferases of methanogenesis from monomethylamine and determination of transcript start sites. Cloning and expression of MtmB in Escherichia coli strain DH5alpha |
| 2.1.1.248 | gene mtmB1, DNA and amino acid sequence determination and analysis, UAG is not the stop codon in the encoding gene, no UAG modification. The mtmB1 amber codon encodes pyrrolysine in UAG, crystal structure of MtmB reveals lysine, but with epsilonN in amide linkage with (4R,5R)-4 substituted-pyrroline-5-carboxylate |
| 2.1.1.248 | gene mtmB1and mtmB2C2, operon encoding MMA methylamine methyltransferase, quantitative RT-PCR expression analysis, comparison with other methylamine methyltransferases, overview. Transcriptional regulation of genes encoding methylamine methyltransferases in cells growing on Ttrimethyamine or methanol in the presence of ammonium, overview |
