EC Number   |
|---|
   2.1.1.240 | expression in Escherichia coli. Transient coexpression of ROMT and grapevine stilbene synthase in tobacco (Nicotiana benthamiana) using the agroinfiltration technique results in the accumulation of pterostilbene in tobacco tissues |
| 2.1.1.240 | gene ROMT, quantitative RT-PCR enzyme expression analysis |
| 2.1.1.240 | gene ROMT, recombinant expression in Corynebacterium glutamicum and in Vitis vinifera cell culture, coexpression with flavanone 3-hydroxylase, and flavonol synthase, transient recombinant expression of HA-tagged enzyme in Nicotiana benthamiana leaves via Agrobacterium-mediated transient transformation, coexpression with human cytochrome P450 hydroxylase 1B1 (HsCYP1B1) |
| 2.1.1.240 | gene ROMT, recombinant expression in Escherichia coli strain BW27784 and in Saccharomyces cerevisiae, coexpression of two additional genes from the resveratrol biosynthetic pathway, 4-coumarate CoA-ligase and stilbene synthase, leads to production of a large amount of pterostilbene with a trace amount of pinostilbene, subcloning in Escherichia coli strain DH5alpha, recombinant expression of grape SUMO-fusion wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) |
| 2.1.1.240 | gene ROMT, recombinant expression of HA-tagged enzyme in Nicotiana tabacum strain SR1 via Agrobacterium tumefaciens strain LBA4404 transformation method, coexpression with genes RpSTS encoding stilbene synthase from Rheum palmatum cv. Jangyeop, real-time quantitative RT-PCR enzyme expression analysis |
| 2.1.1.240 | gene sbOMT3, recombinant expression of codon-optimized resveratrol O-methyltransferase genes sbOMT1 and sbOMT3 in Escherichia coli strain C41 (DE3), development of an artificial biosynthetic pathway that produces methylated resveratrol analogues, such as pinostilbene (3,4'-dihydroxy-5-methoxystilbene), 3,5-dihydroxy-4'-methoxystilbene, 3,4'-dimethoxy-5-hydroxystilbene, and 3,5,4'-trimethoxystilbene, from simple carbon sources in Escherichia coli |
| 2.1.1.240 | gene SbROMT3syn, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, subcloning in Escherichia coli strain DH5alpha |
| 2.1.1.240 | gene VrROMTsyn, recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, subcloning in Escherichia coli strain DH5alpha. Recombinantly expressed enzyme from grape produces very small amounts of methylated resveratrol derivatives in Escherichia coli |
| 2.1.1.240 | recombinant expression in Escherichia coli strains BL21(DE3) and P1, coexpression with genes encoding Saccharothrix espanaensis TAL, Nicotiana tabacum CCL, and Vitis vinifera STS |
| 2.1.1.240 | recombinant expression of the His-tagged enzyme in Escherichia coli strain BL21-CodonPlus (DE3)-RIPL, coexpression with His-tagged cinnamate/4-coumarate:coenzyme A ligase from Streptomyces coelicolor and Strep-tagged stilbene synthase from Rheum palmatum |
