EC Number   |
|---|
    2.1.1.156 | DNA and amino acid sequence determination, functional coexpression in Escherichia coli with sarcosine dimethylglycine methyltransferase, EC 2.1.1.157, conferring betaine accumulation and increased salt tolerance to the Escherichia coli cells |
| 2.1.1.156 | DNA and amino acid sequence determination, the single gene encodes the glycine sarcosine methyltransferase as well as the sarcosine dimethylglycine methyltransferase, EC 2.1.1.157, the latter being probably a proteolytic processing product of the first, poor expression in Escherichia coli of the full length and the truncated enzyme |
| 2.1.1.156 | expressed as soluble His-tag fusion protein in Escherichia coli BL21(DE3)pLysS |
| 2.1.1.156 | expressed in Arabidopsis thaliana |
| 2.1.1.156 | expressed in Escherichia coli BL21(DE3) |
| 2.1.1.156 | expressed in Oryza sativa cultivar Nipponbare |
| 2.1.1.156 | expressed in Zea mays |
| 2.1.1.156 | functional overexpression in Escherichia coli strain XL-1 Blue conferring betaine accumulation and increased salt tolerance when coexpressed with the sarcosine dimethylglycine methyltransferase, EC 2.1.1.157 |
| 2.1.1.156 | gene gsmA, DNA and amino acid sequence determination and analysis, sequence comparison |
| 2.1.1.156 | gene Mpgsmt, DNA and amino acid sequence determination and analysis and sequence comparisons, phylogenetic analysis of GSMT, expression of complete gene cluster of Mpgsmt-sdmt in Escherichia coli strain BL21(DE3) and in strain MKH13 deficient in betaine transport, the recombinant enzyme is functional to synthesize and accumulate betaine and confers elevated survival ability in betaine transport deficient mutant strain MKH13 under high salt stress. The recombinantly expressed enzyme shows 10000fold lower catalytic efficiency compared with the enzyme isolated from the original cells |
