EC Number |
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2.1.1.128 | canadine-producing Saccharomyces cerevisiae strain harbors expression cassettes for seven heterologous enzymes: Papaper somniferum norcoclaurine 6-O-methyltransferase (Ps6OMT), Papaver somniferum 3'-hydroxy-N-methylcoclaurine 4'-O-methyltransferase 2 (Ps4'OMT), Papapver somniferum coclaurine N-methyltransferase (PsCNMT), Papaver somniferum berberine bridge enzyme (PsBBE), Thalictrum flavum scoulerine 9-O-methyltransferase (TfS9OMT), Thalictrum flavum canadine synthase (TfCAS), and Arabidopsis thaliana cytochrome P450 reductase 1 (CPR). The expression cassettes for the methyltransferases Ps6OMT, PsCNMT, and Ps4'OMT and the cytochrome P450 reductase CPR were chromosomally integrated, TfS9OMT and TfCAS are expressed from a high-copy plasmid, and PsBBE is expressed from a second high-copy plasmid |
2.1.1.128 | expressed as His-tag fusion protein in Escherichia coli ER2566pLys S |
2.1.1.128 | expressed in Escherichia coli |
2.1.1.128 | expressed in Escherichia coli BL21(DE3) cells |
2.1.1.128 | expressed in Eschscholzia californica |
2.1.1.128 | expression in Escherichia coli |
2.1.1.128 | full-length cDNA of Coptis japonica 6OMT is cloned into the binary vector pBITXEl2 for introduction into Agrobacterium tumefaciens, for infection Eschscholzia californica seedlings are co-cultured with Agrobacterium tumefaciens |
2.1.1.128 | into the pET-21d vector for expression in Escherichia coli BL21DE3 cells |