EC Number |
---|
1.9.6.1 | - |
1.9.6.1 | cloning of napABC, quantitative PCR-based expression analysis |
1.9.6.1 | expressed in Escherichia coli |
1.9.6.1 | gene nap and nap gene cluster, genetic organization and sequence comparisons |
1.9.6.1 | gene nap, expression analysis |
1.9.6.1 | gene napA, enzyme NapA is encoded, along with its periplasmic di-heme c-type cytochrome redox partner NapB, in the seven gene nap operon, coexpression of NapA with His-tagged NapD activator in Escherichia coli strains MC4100 and BL21(DE3), recombinant expression of MTSL-labelled MalE-NapASP fusion mutant S4C/S24C in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain LCB2048 |
1.9.6.1 | structural genes, napA and napB, are cloned, and their nucleotide sequences is determined |