EC Number |
---|
1.8.1.2 | alpha and beta subunits |
1.8.1.2 | expressed in LMG194 Escherichia coli cells |
1.8.1.2 | FMN-binding domain of flavoprotein component |
1.8.1.2 | gene MET10, DNA and amino acid sequence determination and analysis |
1.8.1.2 | homology to cysI of Escherichia coli, i.e. alpha subunit |
1.8.1.2 | NADPH- and FAD-binding domain of flavoprotein component |
1.8.1.2 | the MET5 and Met10 coding sequences are cloned into the vector pGEM-T-easy and subsequently into the centromeric Saccharomyces cerevisiae expression plasmid pRS416 |
1.8.1.2 | the sulfite reductase hemo-subunit is cloned into the vector pLM1 for expression in Escherichia coli BL21DE3 cells |