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Results 1 - 7 of 7
EC Number Cloned (Commentary)
Show all pathways known for 1.5.8.4Display the word mapDisplay the reaction diagram Show all sequences 1.5.8.4development of an intracellular, heterologous expression system in Komagataella phaffii (formerly known as Pichia pastoris). Initial attempts to express the gene in Escherichia coli (BL21 DE3) yielded largely insoluble protein. The H109R variant is expressed in lower amounts compared to the wild-type enzyme
Show all pathways known for 1.5.8.4Display the word mapDisplay the reaction diagram Show all sequences 1.5.8.4expressed in Escherichia coli BL21(DE3) cells as a C-terminally 6-His-tagged fusion protein which is predominantly in its apo form
Show all pathways known for 1.5.8.4Display the word mapDisplay the reaction diagram Show all sequences 1.5.8.4expressed in Komagataella phaffii KM71H cells
Show all pathways known for 1.5.8.4Display the word mapDisplay the reaction diagram Show all sequences 1.5.8.4expression in Escherichia coli
Show all pathways known for 1.5.8.4Display the word mapDisplay the reaction diagram Show all sequences 1.5.8.4expression in Komagataella phaffii, i.e. Pichia pastoris
Show all pathways known for 1.5.8.4Display the word mapDisplay the reaction diagram Show all sequences 1.5.8.4overexpression in Escherichia coli strain JM109 as N-terminally His-tagged enzyme being equally distributed in the soluble fraction and the insoluble inclusion body fraction
Show all pathways known for 1.5.8.4Display the word mapDisplay the reaction diagram Show all sequences 1.5.8.4screening of a cDNA library prepared in Escherichia coli from rat liver polyA RNA in the plasmid vector peD-X
Results 1 - 7 of 7