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EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 1.5.1.23cloning of the cDNA by PCR. DNA encoding TaDH is amplified by PCR from Suberites domuncula cDNA library, using the primers that are designed to amplify the complete open reading frame. Expression in Escherichia coli as His-tag fusion protein. Two allelic variants are identified, which are present in the different specimens either in a homozygotic or in a heterozygotic way (length polymorphism (453 bp and 468 bp long) is detected in the second intron and the beginning of third exon). 688792
Display the word mapDisplay the reaction diagram Show all sequences 1.5.1.23DNA and amino acid sequence determination and analysis, sequence comparisons 655275
Display the word mapDisplay the reaction diagram Show all sequences 1.5.1.23genotyping of different populations and proteomic analysis, overview 742358
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