EC Number   |
|---|
    1.5.1.10 | - |
| 1.5.1.10 | cloned in Escherichia coli |
| 1.5.1.10 | expressed in Escherichia coli |
| 1.5.1.10 | expressed in Escherichia coli BL21/(DE-3) RIL cells |
| 1.5.1.10 | gene lys, subcloning in Escherichia coli |
| 1.5.1.10 | gene LYS9 is organized as a chimeric spermidine synthase-saccharopine dehydrogenase gene (SPE3-LYS9) encoding functional spermidine synthase, EC 2.5.1.16, and saccharopine dehydrogenase, DNA and amino acid sequence determination and analysis, expression of wild-type enzyme in Saccharomyces cerevisiae, the chimeric gene can complement a Saccharomyces cerevisiae lys9 mutant, but not a spe3 mutant |
| 1.5.1.10 | gene nSpe-Sdh, encoding a chimeric bifunctional gene with a single transcript that also encodes saccharopine dehydrogenase (SDH), DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 |
| 1.5.1.10 | recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain LYS 9 |
