EC Number   |
|---|
  1.3.99.31 | expression in Escherichia coli |
| 1.3.99.31 | expression in Escherichia coli. Synthesis of the enzyme by different vectors with high and lower copy numbers or strong and weak promoters results in variable ratios of lycopene and neurosporene formation. The highest amount of enzyme is produced with pUC vectors with high copy number and a strong promoter. This transformant synthesizes the highest lycopene amount. The lowest enzyme concentrations is found with pPEU carrying a weak promoter. In this transformant lycopene formation is minor. With increased enzyme concentration, a proportion of lycopene higher than in Rubrivivax gelatinosus cells can be obtained |
| 1.3.99.31 | gene carB, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli |
| 1.3.99.31 | gene crtI, cloning of cluster crtCDEF |
| 1.3.99.31 | gene crtI, cloning of the crt gene cluster crtCDEF, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 1.3.99.31 | gene crtI, DNA and amino acid sequence determination and analysis, recombinant enzyme expression in Escherichia coli strain BL21(DE3) |
| 1.3.99.31 | gene crtI, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant enzyme expression from plasmid pCcrt1 in Escherichia coli. Lycopene biosynthesis is confirmed when the plasmid pCcrtI is cotransformed into Escherichia coli containing the plasmid pRScrtEB carrying the crtE and crtB genes required for lycopene biosynthesis |
| 1.3.99.31 | gene crtI, recombinant coexpression of genes crtE, crtB, and crtI from Erwinia uredovora and of crtL from Ficus carica in Pichia pastoris X-33 strains for large-scale biosynthesis of carotenoids, subcloning of crtI in Escherichhia coli strain TOP10 |
| 1.3.99.31 | gene crtI, recombinant expression of His6-tagged enzyme in Escherichia coli |
| 1.3.99.31 | gene crtI, recombinant expression of the enzyme driven by 35S promoter in via Agrobacterium tumefaciens strain GV 3101 containing binary vector pRI 101(I) in fruits of Solanum tuberosum, coexpression of genes CrtI and CrtY, the latter encoding lycopene beta-cyclase, in transgenic tomato fruits, the method used is agroinjection of tomato fruits, a method of directly injecting Agrobacterium containing transgenes into fruits |
