EC Number   |
|---|
  1.3.99.30 | expression in Escherichia coli |
| 1.3.99.30 | expression in Escherichia coli. Generation of a 3,4-didehydrolycopene accumulating Escherichia coli strain |
| 1.3.99.30 | gene al-1, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli |
| 1.3.99.30 | gene carB, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli |
| 1.3.99.30 | overexpression in Xanthophyllomyces dendrorhous. To achieve this, the plasmids pPR40F and pPR40R are introduced in Xanthophyllomyces dendrorhous CBS 6938. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products |
