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EC Number Cloned (Commentary)
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98expressed in Escherichia coli and in host strain
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98expressed in Escherichia coli BL21(DE3) cells
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98expressed in Escherichia coli DH5alpha cells
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98expression of MurB in Escherichia coli strain BL21(DE3)
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98gene murB or alr5066, construction of plasmid pPJAV308 and recombinant expression of the enzyme in Escherichia coli, functional complementation of a MurB enzyme-deficient Escherchia coli mutant
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98gene murB, expression of His-tagged enzyme in Escherichia coli K12S
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98gene murB, overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), functional complementation of murB-deficient, temperature-sensitive Staphylcoccus aureus mutant strain TS2901 by the wild-type enzyme, and enzyme mutants G67A, S70A, G69A, and R230A
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98gene murB, recombinant expression of N-terminally His6-tagged enzyme, harboring a TEV protease cleavage site, in Escherichia coli strain BL21(DE3)
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98gene murB, Verrucomicrobium spinosum possesses a fusion open reading frame annotated by the locus tag (VspiD_010100018130). Recombinant expression in Escherichia coli strain Rosetta (DE3) pLysS. The ORF, which is predicted to encode the enzymes UDP-N-acetylenolpyruvoylglucosamine reductase (MurB) and UDP-N-acetylmuramate:L-alanine ligase (MurC) is cloned. In vivo analyses using functional complementation shows that the fusion gene is able to functionally complement Escherichia coli murB (mutant ST5 strain) and murC temperature sensitive mutants. The purified recombinant fusion enzyme (MurB/CVs) is endowed with UDP-N-acetylmuramate:L-alanine ligase activity. All attempts to demonstrate an in vitro UDP-N-acetylenolpyruvoylglucosamine reductase (MurB) activity are unsuccessful. Phylogenetic analysis reveals that this fusion enzyme can only be identified in specific lineages within the Verrucomicrobia phylum. The fusion gene is able to functionally complement two Escherichia coli strains that harbor mutations in the murB and murC genes
Show all pathways known for 1.3.1.98Display the word mapDisplay the reaction diagram Show all sequences 1.3.1.98overexpression in Escherichia coli XA90F'lacIQ1
Results 1 - 10 of 13 > >>