EC Number   |
Reference |
|---|
    1.3.1.72 | construction of a recombinant adenovirus driving DHCR24 expression vector specific for neurons with neuron-specific promoter, synapsin-1 (SYN1), cloning of a SYN1 promoter DNA fragment of human enzyme DHCR24, and transfection of recombinant Ad-hSYN1-DHCR24 into HEK AD-293, N2A (mouse neuroblastoma), and MIN6 (mouse pancreatic carcinoma) cells. DHCR24 is specially expressed in HEK AD-293 and N2A cells, but not in MIN6 cells. Adenovirus transfection inhibits apoptosis through scavenging excess reactive oxygen species, and recombinant DHCR24 adenoviruse induces neuron-specific DHCR24 expression |
745890 |
| 1.3.1.72 | construction of a recombinant adenovirus driving DHCR24 expression vector specific for neurons with neuron-specific promoter, synapsin-1 (SYN1), cloning of a SYN1 promoter DNA fragment of rat enzyme DHCR24, and transfection of recombinant Ad-rSYN1-DHCR24 into HEK AD-293, N2A (mouse neuroblastoma), and MIN6 (mouse pancreatic carcinoma) cells. DHCR24 is specially expressed in HEK AD-293 and N2A cells, but not in MIN6 cells. Adenovirus transfection inhibits apoptosis through scavenging excess reactive oxygen species, and recombinant DHCR24 adenoviruse induces neuron-specific DHCR24 expression |
745890 |
| 1.3.1.72 | DWF1, phylogenetic tree |
746354 |
| 1.3.1.72 | ectopic expression of wild-type and mutant human DHCR24s in CHO-7 cells that are deficient for DHCR24 through specific siRNA knockout, quantitative real-time RT-PCR enzyme expression analysis |
745512 |
| 1.3.1.72 | expressed as a EGFP-fusion protein in murine neuroblastoma cell line N2A |
725752 |
| 1.3.1.72 | expressed in CHO cells |
724442 |
| 1.3.1.72 | expressed in HEK-293 cells |
764328 |
| 1.3.1.72 | expressed in Mus musculus embryonic fibroblasts and PC-12 cells infected with adenovirus |
686416 |
| 1.3.1.72 | gene ArDWF1, cloning from hairy root expression sequence tag library, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, functional recombinant expression in Saccharomyces cerevisiae, ArDWF1-expressing yeast T21 cells produce 24-methylcholesterol in vivo |
745361 |
| 1.3.1.72 | gene DHCR24, DNA and amino acid sequence determination and analysis of wild-type and natural desmosterolysis mutant genes, chromosomal mapping to 1p31.1-p33, functional expression of wild-type and mutant enzymes in Saccharomyces cerevisiae |
654165 |
